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r3101s  (New England Biolabs)


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    Structured Review

    New England Biolabs r3101s
    R3101s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori+hf/EcoRI-HF/pmc13125920-17-7-2
    Average 99 stars, based on 2123 article reviews
    r3101s - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism
    Article Snippet: .. The neutral site 1 (NSI) plasmid pAM2314 (Ditty et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and EcoRI digested pAM2314 plasmid DNA products were gel purified and Gibson assembled (New England Biolabs) to create the pAM2341-PglgA:: glgA-eyfp (pMR0300) plasmid.

    Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism
    Article Snippet: .. The neutral site 1 (NSI) plasmid pAM2991 (Ivleva et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and EcoRI digested pAM2991 plasmid DNA products were gel purified and Gibson assembled (New England Biolabs) to create the pAM2991-Ptrc::glgA-EYFP (pMR0301) plasmid.

    Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.
    Article Snippet: .. 4 of 24 1 fused to Maroon1 from pLL3.7m-mTurquoise2-SLBP was eliminated by excision of the vector with EcoRI-HF (NEB, R3101S). .. After gel purification using the QIAquick Gel Extraction Kit (Qiagen, 28706), the resulting cut vector was religated overnight with T4 ligase (NEB, M0202S).

    Polymerase Chain Reaction:

    Article Title: Nanoplate based digital PCR assay for effective quantification of plasma HPV circulating tumor DNA.
    Article Snippet: .. The reaction contained 10 μL of 4× Probe PCR Master Mix, 2 μL each of forward-primer and reverse-primer pools, 4 μL of probe pool, 1 μL of EcoRI-HF® (NEB®) restriction enzyme, 2 μL of template mix, and nuclease-free water to make reaction up to 40 μL. ..

    Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.
    Article Snippet: .. The obtained vectors were cut with AscI (New England Biolabs; # R0558S) and EcoRI-HF (New England Biolabs; # R3101S) and were purified with QIAquick PCR & st). (D) Representative images showing the intensity and distribution of R and HA-tagged PIP5K1A wt, NLS or NES variants. shNTC and HAspectively. ..

    Purification:

    Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation.
    Article Snippet: .. The obtained vectors were cut with AscI (New England Biolabs; # R0558S) and EcoRI-HF (New England Biolabs; # R3101S) and were purified with QIAquick PCR & st). (D) Representative images showing the intensity and distribution of R and HA-tagged PIP5K1A wt, NLS or NES variants. shNTC and HAspectively. ..



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